Author:
Song Daesub,Ha Gunwoo,Serhan Wissam,Eltahir Yassir,Yusof Mohammed,Hashem Farouq,Elsayed Elsaeid,Marzoug Bahaaeldin,Abdelazim Assem,Al Muhairi Salama
Abstract
We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.
Publisher
American Society for Microbiology
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