Affiliation:
1. Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston 77555-1019.
Abstract
Salmonella infection of the gastrointestinal tract (GT) results in fluid secretion and inflammation. In contrast, cholera toxin (CT) induces fluid secretion but no inflammation. Using a murine ligated intestinal loop model, we investigated cytokine production (interleukin-1 [IL-1], IL-2, IL-4, IL-6, IL-10, gamma interferon, and tumor necrosis factor alpha) in the GT following exposure to these agents. Salmonella typhimurium induced a Th1-like cytokine profile in loops obtained from either nonimmune mice or Salmonella-immunized mice. CT induced only IL-6 and IL-10 production in ligated loops from nonimmune mice but induced a Th2-like cytokine profile in ligated loops obtained from CT-immunized mice. These results show that CT and S. typhimurium induce very different cytokine profiles in the GT.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference21 articles.
1. Tumor necrosis factor (TNF~) regulates intestinal mucus production during salmonellosis;Arnold J. W.;Cell Immunol.,1993
2. Tumor necrosis factormediates the early pathology in Salmonella infection of the gastrointestinal tract;Arnold J. W.;Microb. Pathog.,1993
3. Dertzbaugh M. T. and C. O. Elson. 1991. Cholera toxin as a mucosal adjuvant p. 119-131. In D. R. Spriggs and W. C. Koff (ed.) Topics in vaccine adjuvant research. CRC Press Inc. Boca Raton Fla.
4. IL10 inhibits mitogen-induced T cell proliferation by selectively inhibiting macrophage costimulatory function;Ding L.;J. Immunol.,1992
5. Differential cytokine expression by human intestinal epithelial cell lines: regulated expression of IL8;Eckmann L.;Gastroenterology,1993