Growth of Thiobacillus ferrooxidans on Formic Acid

Author:

Pronk J. T.1,Meijer W. M.1,Hazeu W.1,van Dijken J. P.1,Bos P.1,Kuenen J. G.1

Affiliation:

1. Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands

Abstract

A variety of acidophilic microorganisms were shown to be capable of oxidizing formate. These included Thiobacillus ferrooxidans ATCC 21834, which, however, could not grow on formate in normal batch cultures. However, the organism could be grown on formate when the substrate supply was growth limiting, e.g., in formate-limited chemostat cultures. The cell densities achieved by the use of the latter cultivation method were higher than cell densities reported for growth of T. ferrooxidans on ferrous iron or reduced sulfur compounds. Inhibition of formate oxidation by cell suspensions, but not cell extracts, of formate-grown T. ferrooxidans occurred at formate concentrations above 100 μM. This observation explains the inability of the organism to grow on formate in batch cultures. Cells grown in formate-limited chemostat cultures retained the ability to oxidize ferrous iron at high rates. Ribulose 1,5-bisphosphate carboxylase activities in cell extracts indicated that T. ferrooxidans employs the Calvin cycle for carbon assimilation during growth on formate. Oxidation of formate by cell extracts was NAD(P) independent.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference30 articles.

1. The relationship between chemiosmotic parameters and sensitivity to anions and organic acids in the acidophile Thiobacillus ferrooxidans;Alexander B.;J. Gen. Microbiol.,1987

2. The molar extinction coefficient of 2,6-dichlorophenol indophenol;Armstrong J. M.;Biochim. Biophys. Acta,1964

3. Atkins A. S. 1978. Studies on the oxidation of sulphide minerals (pyrite) in the presence of bacteria p. 403-426. In L. E. Murr A. E. Torma and J. A. Brierley (ed.) Metallurgical applications of bacterial leaching and related microbiological phenomena. Academic Press Inc. New York.

4. Relations between D-ribulose-1,5-bisphosphate carboxylase, carboxysomes and C02-fixing capacity in the obligate chemolithotroph Thiobacillus neapolitanus grown under energy limitation in the chemostat;Beudeker R. F.;Arch. Microbiol.,1980

5. Blake R. C. E. A. Shute and K. J. White. 1989. Enzymology of respiratory iron oxidation p. 391-401. In J. Salley R. G. L. McCready and P. L. Wichlacz (ed.) Proceedings of the International Symposium of Biohydrometallurgy. Jackson Hole Wyoming 1989. Canada Centre for Mineral and Energy Technology.

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