Deletion Mutants, Archived Transposon Library, and Tagged Protein Constructs of the Model Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough

Author:

Wall Judy D.1,Zane Grant M.1,Juba Thomas R.1,Kuehl Jennifer V.2,Ray Jayashree3,Chhabra Swapnil R.3,Trotter Valentine V.4,Shatsky Maxim2,De León Kara B.1,Keller Kimberly L.1,Bender Kelly S.1,Butland Gareth4,Arkin Adam P.2,Deutschbauer Adam M.2

Affiliation:

1. Biochemistry Division, University of Missouri, Columbia, Missouri, USA

2. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

3. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

4. Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

Abstract

The dissimilatory sulfate-reducing deltaproteobacterium Desulfovibrio vulgaris Hildenborough (ATCC 29579) was chosen by the research collaboration ENIGMA to explore tools and protocols for bringing this anaerobe to model status. Here, we describe a collection of genetic constructs generated by ENIGMA that are available to the research community.

Funder

U.S. Department of Energy

University of Missouri

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

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