Author:
Wovcha M G,Steuerwald D L,Brooks K E
Abstract
A recombinant plasmid, designated pUC1002, was constructed by ligation of a HindIII restriction endonuclease fragment of Escherichia coli chromosomal DNA to vector plasmid pMB9. Strains carrying this plasmid were selected by transformation of an E. coli strain bearing the xyl-7 mutation to a xylose-positive (Xyl+) phenotype. Strains containing pUC1002 produced coordinately elevated levels of D-xylose isomerase and D-xylulose kinase. Under appropriate conditions, the isomerase also efficiently catalyzed the conversion of D-glucose to D-fructose.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference23 articles.
1. Colorimetric analysis of sugars;Ashwell G.;Methods Enzymol.,1957
2. D-Xylulose kinase from calf liver;Ashwell G.;Methods Enzymol.,1962
3. Construction and characterization of new cloning vehicles. 1. Ampicillin-resistant derivatives of the plasmid pMB9;Bolivar F.;Gene,1977
4. Bucke C. 1977. Industrial glucose isomerase p. 147-171. In A. Wiseman (ed.) Topics in enzyme and fermentation biotechnology part B. Halstead Press Chichester England.
5. D-Xylulose fermentation to ethanol by Saccharomyces cerevisiae;Chiang L.;Appl. Environ. Microbiol.,1981
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