Transcriptional Regulation of Plant Biomass Degradation and Carbohydrate Utilization Genes in the Extreme Thermophile Caldicellulosiruptor bescii

Author:

Rodionov Dmitry A.12ORCID,Rodionova Irina A.3ORCID,Rodionov Vladimir A.2,Arzamasov Aleksandr A.12,Zhang Ke4,Rubinstein Gabriel M.5,Tanwee Tania N. N.5,Bing Ryan G.6,Crosby James R.6,Nookaew Intawat78,Basen Mirko9ORCID,Brown Steven D.8,Wilson Charlotte M.810,Klingeman Dawn M.8,Poole Farris L.5,Zhang Ying4ORCID,Kelly Robert M.6,Adams Michael W. W.5ORCID

Affiliation:

1. Sanford-Burnhams-Prebys Medical Discovery Institute, La Jolla, California, USA

2. A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia

3. Department of Bioengineering, University of California—San Diego, La Jolla, California, USA

4. Department of Cell and Molecular Biology, College of the Environment and Life Sciences, University of Rhode Island, Kingston, Rhode Island, USA

5. Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA

6. Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA

7. Department of Biomedical Informatics, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

8. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

9. Mathematisch-Naturwissenschaftliche Fakultät, Institut für Biowissenschaften, Mikrobiologie, Universität Rostock, Rostock, Germany

10. University of Otago, Dunedin, New Zealand

Abstract

To develop functional metabolic engineering platforms for nonmodel microorganisms, a comprehensive understanding of the physiological and metabolic characteristics is critical. Caldicellulosiruptor bescii and other species in this genus have untapped potential for conversion of unpretreated plant biomass into industrial fuels and chemicals. The highly interactive and complex machinery used by C. bescii to acquire and process complex carbohydrates contained in lignocellulose was elucidated here to complement related efforts to develop a metabolic engineering platform with this bacterium.

Funder

HHS | NIH | National Institute of General Medical Sciences

U.S. Department of Energy

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modelling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

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