Single-Fluorescence ATP Sensor Based on Fluorescence Resonance Energy Transfer Reveals Role of Antibiotic-Induced ATP Perturbation in Mycobacterial Killing-Reference-Cited by-同舟云学术

Single-Fluorescence ATP Sensor Based on Fluorescence Resonance Energy Transfer Reveals Role of Antibiotic-Induced ATP Perturbation in Mycobacterial Killing

Published:2022-06-28 Issue:3 Volume:7 Page:
ISSN:2379-5077
Container-title:mSystems
language:en
Short-container-title:mSystems

Author:

Liang Lujie¹²,Lin Daixi¹²,Chen Yishen³,Li Jiachen¹²,Liang Wanfei¹²,Zhao Hui⁴,Luo Wenji³,Tian Guo-bao¹²^ORCID,Feng Siyuan¹²^ORCID

Affiliation:

1. Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China

2. Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, China

3. Department of Pharmacy, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China

4. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China

Abstract

Bioenergetics has emerged as a new paradigm for antituberculosis (anti-TB) drug discovery, and the cellular ATP level is the core indicator reflecting bacterial metabolic homeostasis. Although several bulk assays have been designed for the measurement of cellular ATP content, a more convenient strategy is required for real-time ATP measurement of single viable cells.

Funder

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modeling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/msystems.00209-22

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