A Combination of Metagenomic and Cultivation Approaches Reveals Hypermutator Phenotypes within Vibrio cholerae-Infected Patients-Reference-Cited by-同舟云学术

A Combination of Metagenomic and Cultivation Approaches Reveals Hypermutator Phenotypes within Vibrio cholerae-Infected Patients

Published:2021-08-31 Issue:4 Volume:6 Page:
ISSN:2379-5077
Container-title:mSystems
language:en
Short-container-title:mSystems

Author:

Levade Inès¹²,Khan Ashraful I.³,Chowdhury Fahima³,Calderwood Stephen B.⁴⁵⁶,Ryan Edward T.⁴⁵,Harris Jason B.⁴⁷,LaRocque Regina C.⁴⁵,Bhuiyan Taufiqur R.³^ORCID,Qadri Firdausi³,Weil Ana A.⁸,Shapiro B. Jesse¹²⁹^ORCID

Affiliation:

1. Department of Biological Sciences, University of Montreal, Montreal, Quebec, Canada

2. Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada

3. Center for Vaccine Sciences, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh

4. Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA

5. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA

6. Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA

7. Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA

8. Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA

9. McGill Genome Centre, Montreal, Quebec, Canada

Abstract

Pathogen evolution within patients can impact phenotypes such as drug resistance and virulence, potentially affecting clinical outcomes. V. cholerae infection can result in life-threatening diarrheal disease or asymptomatic infection. Here, we describe whole-genome sequencing of V. cholerae isolates and culture-free metagenomic sequencing from stool of symptomatic cholera patients and asymptomatic carriers.

Funder

Gouvernement du Canada | Canadian Institutes of Health Research

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modeling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSystems.00889-21

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5. Cholera

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