A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis-Reference-Cited by-同舟云学术

A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis

Published:2021-10-26 Issue:5 Volume:6 Page:
ISSN:2379-5077
Container-title:mSystems
language:en
Short-container-title:mSystems

Author:

Song Fangchao¹,Kuehl Jennifer V.¹,Chandran Arjun²,Arkin Adam P.¹²

Affiliation:

1. Environmental Genomics & Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

2. Department of Bioengineering, University of California at Berkeley, Berkeley, California, USA

Abstract

Understanding bacterial interactions and assembly in complex microbial communities using 16S rRNA sequencing normally requires a large experimental load. However, the current DNA extraction methods, including cell disruption and genomic DNA purification, are normally biased, costly, time-consuming, labor-intensive, and not amenable to miniaturization by droplets or 1,536-well plates due to the significant DNA loss during the purification step for tiny-volume and low-cell-density samples.

Funder

U.S. Department of Energy

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modelling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSystems.00224-21

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