Transcriptional Variation of Diverse Enteropathogenic Escherichia coli Isolates under Virulence-Inducing Conditions

Author:

Hazen Tracy H.12,Daugherty Sean C.1,Shetty Amol C.1,Nataro James P.3,Rasko David A.12ORCID

Affiliation:

1. Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA

2. Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA

3. Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, Virginia, USA

Abstract

Recent studies have demonstrated that there is considerable genomic diversity among EPEC isolates; however, it is unknown if this genomic diversity leads to differences in their global transcription. This study used RNA-Seq to compare the global transcriptomes of EPEC isolates from diverse phylogenomic lineages. We demonstrate that there are lineage- and isolate-specific differences in the transcriptomes of genomically diverse EPEC isolates during growth under in vitro virulence-inducing conditions. This study addressed biological variation among isolates of a single pathovar in an effort to demonstrate that while each of these isolates is considered an EPEC isolate, there is significant transcriptional diversity among members of this pathovar. Future studies should consider whether this previously undescribed transcriptional variation may play a significant role in isolate-specific variability of EPEC clinical presentations.

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modeling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

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