Quantitative Stable-Isotope Probing (qSIP) with Metagenomics Links Microbial Physiology and Activity to Soil Moisture in Mediterranean-Climate Grassland Ecosystems

Author:

Greenlon Alex1,Sieradzki Ella1,Zablocki Olivier23,Koch Benjamin J.45,Foley Megan M.45,Kimbrel Jeffrey A.6,Hungate Bruce A.45ORCID,Blazewicz Steven J.6,Nuccio Erin E.6,Sun Christine L.23,Chew Aaron1,Mancilla Cynthia-Jeanette1,Sullivan Matthew B.237,Firestone Mary1ORCID,Pett-Ridge Jennifer68ORCID,Banfield Jillian F.19ORCID

Affiliation:

1. Department of Environmental Science, Policy and Management, University California, Berkeley, Berkley, California, USA

2. Department of Microbiology, Ohio State University, Columbus, Ohio, USA

3. Center of Microbiome Science, Ohio State University, Columbus, Ohio, USA

4. Center for Ecosystem Science and Society, Northern Arizona University, Flagstaff, Arizona, USA

5. Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, USA

6. Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, California, USA

7. Department of Civil, Environmental and Geodetic Engineering, Ohio State University, Columbus, Ohio, USA

8. Life & Environmental Sciences Department, University of California, Merced, Merced, California, USA

9. Department of Earth and Planetary Science, University of California, Berkeley, Berkley, California, USA

Abstract

Soil moisture is a critical factor that strongly shapes the lifestyle of soil organisms by changing access to nutrients, controlling oxygen diffusion, and regulating the potential for mobility. We identified active microorganisms in three grassland soils with similar mineral contexts, yet different historic rainfall inputs, by adding water labeled with a stable isotope and tracking that isotope in DNA of growing microbes.

Funder

U.S. Department of Energy

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modeling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

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