DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes-Reference-Cited by-同舟云学术

DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes

Published:2021-10-26 Issue:5 Volume:6 Page:
ISSN:2379-5077
Container-title:mSystems
language:en
Short-container-title:mSystems

Author:

Sorensen Jackson W.¹,Zinke Laura A.¹,ter Horst Anneliek M.¹,Santos-Medellín Christian¹,Schroeder Alena¹,Emerson Joanne B.¹²

Affiliation:

1. Department of Plant Pathology, University of California, Davis, Davis, California, USA

2. Genome Center, University of California, Davis, Davis, California, USA

Abstract

Viromics is becoming an increasingly popular method for characterizing soil viral communities. DNase treatment of the viral size fraction prior to DNA extraction is meant to reduce contaminating free DNA and is a common step within viromics protocols to ensure that sequences are of viral origin.

Funder

U.S. Department of Agriculture

U.S. Department of Energy

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modelling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSystems.00614-21

Reference73 articles.

1. The Gut Virome Database Reveals Age-Dependent Patterns of Virome Diversity in the Human Gut

2. Soil Viruses: A New Hope

3. Patterns and ecological drivers of ocean viral communities

4. Marine viruses — major players in the global ecosystem

5. Phage combination therapies for bacterial wilt disease in tomato

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