Genome-Wide Identification of Tomato Xylem Sap Fitness Factors for Three Plant-Pathogenic Ralstonia Species

Author:

Georgoulis Stratton J.1ORCID,Shalvarjian Katie E.2,Helmann Tyler C.23ORCID,Hamilton Corri D.4,Carlson Hans K.5,Deutschbauer Adam M.25,Lowe-Power Tiffany M.12ORCID

Affiliation:

1. Department of Plant Pathology, University of California Davis, Davis, California, USA

2. Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, California, USA

3. Emerging Pests and Pathogens Research Unit, Robert W. Holley Center, Agricultural Research Service, U.S. Department of Agriculture, Ithaca, New York, USA

4. Department of Plant Pathology, University of Wisconsin—Madison, Madison, Wisconsin, USA

5. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

Abstract

Traditional transposon mutagenesis genetic screens pioneered molecular plant pathology and identified core virulence traits like the type III secretion system. TnSeq approaches that leverage next-generation sequencing to rapidly quantify transposon mutant phenotypes are ushering in a new wave of biological discovery.

Funder

U.S. Department of Agriculture

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modeling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

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