Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology

Author:

Hong JiYoung1,Kang Byunghak1,Kim Ahyoun1,Hwang Seoyoun1,Lee Sunwha1,Kim Jonghyen2,Lee Hyun-Young3,Kang Sang-Hyeon3,Cheon Doo-Sung1

Affiliation:

1. Division of Enteric and Hepatitis Viruses, Center for Infectious Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, 5 Nokbun-dong, Eunpyung-gu, Seoul 122-701, South Korea

2. Department of Pediatrics, Catholic University College of Medicine, Suwon, South Korea

3. iNtRON Biotechnology, Inc., Room 903, JungAng Induspia V, 138-6, Sangdaewon-Dong, Jungwon-gu, Seongnam-si, Gyeonggi-do 462-120, South Korea

Abstract

ABSTRACT To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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