Wnt-1 Induces Growth, Cytosolic β-Catenin, and Tcf/Lef Transcriptional Activation in Rat-1 Fibroblasts-Reference-Cited by-同舟云学术

Wnt-1 Induces Growth, Cytosolic β-Catenin, and Tcf/Lef Transcriptional Activation in Rat-1 Fibroblasts

Published:1998-05 Issue:5 Volume:18 Page:2474-2485
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Young C. S.¹,Kitamura Marina²,Hardy Stephen²,Kitajewski Jan¹

Affiliation:

1. Department of Pathology and Center for Reproductive Sciences, College of Physicians and Surgeons, Columbia University, New York, New York 10032, 1 and

2. Cell Genesys Inc., Foster City, California 944042

Abstract

ABSTRACT Genetic evidence suggests that regulation of β-catenin and regulation of Tcf/Lef family transcription factors are downstream events of the Wnt signal transduction pathway. However, a direct link between Wnt activity and Tcf/Lef transcriptional activation has yet to be established. In this study, we show that Wnt-1 induces a growth response in a cultured mammalian cell line, Rat-1 fibroblasts. Wnt-1 induces serum-independent cellular proliferation of Rat-1 fibroblasts and changes in morphology. Rat-1 cells stably expressing Wnt-1 (Rat-1/Wnt-1) show a constitutive up-regulation of cytosolic β-catenin, while membrane-associated β-catenin remains unaffected. Induction of cytosolic β-catenin in Rat-1/Wnt-1 cells is correlated with activation of a Tcf-responsive transcriptional element. We thus provide evidence that Wnt-1 induces Tcf/Lef transcriptional activation in a mammalian system. Expression of a mutant β-catenin (β-CatS37A) in Rat-1 cells does not result in a proliferative response or a detectable change in the cytosolic β-catenin protein level. However, β-CatS37A expression in Rat-1 cells results in strong Tcf/Lef transcriptional activation, comparable to that seen in Wnt-1-expressing cells. These results suggest that Wnt-1 induction of cytosolic β-catenin may have functions in addition to Tcf/Lef transcriptional activation.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.18.5.2474

Reference54 articles.

1. β-Catenin is a target for the ubiquitin-proteasome pathway;Aberle H.;EMBO J.,1997

2. Functional interaction of beta-catenin with the transcription factor LEF-1;Behrens J.;Nature,1996

3. An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3;Bett A. J.;Proc. Natl. Acad. Sci. USA,1994

4. A new member of the frizzled family from Drosophila functions as a Wingless receptor;Bhanot P.;Nature,1996

5. Alterations of the growth characteristics of the fibroblast cell line C3H10T1/2 by members of the Wnt gene family;Bradbury J. M.;Oncogene,1994

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