Abstract
An inducible beta-oxidation system was demonstrated in a particulate fraction from Neurospora crassa. The activities of all individual beta-oxidation enzymes were enhanced in cells after a shift from a sucrose to an acetate medium. The induction was even more pronounced in transfer to a medium containing oleate as sole carbon and energy source. Since an acyl-coenzyme A (CoA) dehydrogenase was detected instead of acyl-CoA oxidase, the former enzyme seems to catalyze the first step of the beta-oxidation sequence in N. crassa. After isopycnic centrifugation in a linear sucrose gradient, the intracellular organelles housing the fatty acid degradation pathway cosedimented (1.21 g/cm3) with the glyoxylate bypass enzymes isocitrate lyase and malate synthase and were clearly resolved from both mitochondrial marker enzymes (1.19 g/cm3) and catalase (1.26 g/cm3). On the basis of biochemical as well as morphological properties, these particles from N. crassa have recently been designated as glyoxysome-like particles (G. Wanner and T. Theimer, Ann. N.Y. Acad. Sci. 386:269-284, 1982). The failure to detect catalase, urate oxidase, and acyl-CoA oxidase indicate that these glyoxysome-like microbodies in N. crassa lack peroxisomal function and thus are clearly different from the various microbodies reported so far to contain a beta-oxidation pathway.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference30 articles.
1. Aebi H. 1974. Katalase p. 713-718. In H. U. Bergmeyer (ed.) Methoden der Enzymatischen Analyse 3rd ed. Verlag Chemie Weinheim Federal Republic of Germany.
2. Microbodies in higher plants. Annu;Beevers H.;Rev. Plant Physiol.,1979
3. Bergmeyer H. U. K. Gawehn and M. Grassl. 1974. Fumarase p. 479-480. In H. U. Bergmeyer (ed.) Methoden der Enzymatischen Analyse 3rd ed. Verlag Chemie Weinheim Federal Republic of Germany.
4. Localization of some enzymes of p-oxidation of fatty acids in the peroxisomes of Tetrahymena;Blum J. J.;J. Protozol.,1973
5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding;Bradford M. M.;Anal. Biochem.,1976
Cited by
83 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献