Author:
Ohmiya K,Shirai M,Kurachi Y,Shimizu S
Abstract
An enzyme active against p-nitrophenyl-beta-D-glucoside was purified from logarithmic-phase cells of Ruminococcus albus cultivated in a medium containing ball-milled cellulose. The purification yielded homogeneous enzyme after an approximately 520-fold increase in specific activity and a 9% yield. The enzyme was identified as a beta-glucosidase because it can hydrolyze cellobiose and cellooligosaccharides to glucose from the nonreducing ends.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
65 articles.
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