Complete Genome Sequence of Brucella abortus 2308, Isolated from an Abortion Storm on a Dairy Farm in India

Author:

Kumar Amit1ORCID,Prajapati Malyaj R.1,Upadhyay Surendra1,Bhordia Anamika1,Singh Vinod Kumar2,Singh Jitender1,Rahal Anu3,Kumar Ravindra1,Sirohi Anil1

Affiliation:

1. College of Biotechnology, Sardar Vallbhbhai Patel University of Agriculture & Technology, Meerut, Uttar Pradesh, India

2. College of Veterinary Sciences, UP Pt. Deen Dayal Uphadhayay Pashu Chikitsa Vigyan Viswavidhyalay Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India

3. Division of Animal Health, ICAR—Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India

Abstract

The present report communicates the first complete genome sequence of Brucella abortus strain 2308, isolated from an abortion storm on a dairy farm in India. Bacteria were isolated in pure culture from the placentas of aborted calves, and next-generation sequencing (NGS) revealed that the genome sequence length of the isolated strain is 3,285,606 bp, with a GC content of 57.25%, an N 50 value of 296,426 bp, and an L 50 value of 4, containing 3,119 coding DNA sequences (CDSs), 49 tRNAs, 1 transfer-messenger RNA (tmRNA), and 3 rRNA genes.

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Reference11 articles.

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2. OIE (World Organisation for Animal Health). 2004. Bovine brucellosis. In OIE manual of standards for diagnostic tests and vaccines, 5th ed. OIE, Paris, France.

3. Safety and efficacy of reduced dose Brucella abortus strain 19 vaccine in non-pregnant zebu cattle (Bos indicus) heifers;Kumar A;Indian J Anim Sci,2018

4. Markers for the Molecular Diagnosis of Brucellosis in Animals

5. Brucella abortus Strain 2308 Wisconsin Genome: Importance of the Definition of Reference Strains

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