Electroporation-mediated transfection of Acholeplasma laidlawii with mycoplasma virus L1 and L3 DNA-Reference-Cited by-同舟云学术

Electroporation-mediated transfection of Acholeplasma laidlawii with mycoplasma virus L1 and L3 DNA

Published:1988-08 Issue:8 Volume:62 Page:3050-3052
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Lorenz A¹,Just W¹,da Silva Cardoso M¹,Klotz G¹

Affiliation:

1. Abteilung Virologie, Institut für Mikrobiologie, Universität Ulm, Federal Republic of Germany.

Abstract

In contrast to mycoplasma virus L1 and L2 circular DNA, mycoplasma virus L3 linear DNA is not biologically active in polyethylene glycol-mediated transfection. Electroporation of Acholeplasma laidlawii, however, leads to plaque formation after incubation with L3 DNA. The efficiency of electroporation-mediated transfection is 1/10 that of polyethylene glycol-mediated transfection as estimated with L1 DNA. Trypsin treatment of cells before DNA addition increases the efficiency of DNA uptake.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.62.8.3050-3052.1988

Reference10 articles.

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2. Isolation of Mycoplasmatales virus laidlawii 3, a new virus infecting Acholeplasma laidlawii;Gourlay R. N.;J. Gen. Virol.,1973

3. Structural and biological properties of mycoplasmavirus MVL3: an unusual virus-procaryote interaction;Haberer K.;J. Virol.,1979

4. Infection of Acholeplasma laidlawii by MVL51 virus;Liss A.;Virology,1973

5. Gene transfer into mouse L-cells by electroporation in high electric fields;Neumann E.;EMBO J.,1982

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