IS Ecp1 -Mediated Transposition Leads to Fosfomycin and Broad-Spectrum Cephalosporin Resistance in Klebsiella pneumoniae-Reference-Cited by-同舟云学术

IS Ecp1 -Mediated Transposition Leads to Fosfomycin and Broad-Spectrum Cephalosporin Resistance in Klebsiella pneumoniae

Published:2020-04-21 Issue:5 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Kieffer Nicolas¹,Poirel Laurent¹²³,Mueller Linda¹³,Mancini Stefano¹⁴,Nordmann Patrice¹²³⁵

Affiliation:

1. Medical and Molecular Microbiology, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland

2. INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland

3. Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland

4. Institut für Medizinische Mikrobiologie, Universität Zürich, Zurich, Switzerland

5. Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland

Abstract

A fosfomycin-resistant and carbapenemase (OXA-48)-producing Klebsiella pneumoniae isolate was recovered, and whole-genome sequencing revealed IS Ecp1-bla CTX-M-14b tandemly inserted upstream of the chromosomally encoded lysR-fosA locus. Quantitative evaluation of the expression of lysR and fosA genes showed that this insertion brought a strong hybrid promoter leading to overexpression of the fosA gene, resulting in fosfomycin resistance.

Funder

Université de Fribourg

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00150-20

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