Recombination Can Lead to Spurious Results in Retroviral Transduction with Dually Fluorescent Reporter Genes-Reference-Cited by-同舟云学术

Recombination Can Lead to Spurious Results in Retroviral Transduction with Dually Fluorescent Reporter Genes

Published:2013-12-15 Issue:24 Volume:87 Page:13900-13903
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Salamango Daniel J.¹,Evans David A.¹,Baluyot Mariju F.¹,Furlong Jackson N.¹,Johnson Marc C.¹

Affiliation:

1. Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, Missouri, USA

Abstract

ABSTRACT Fluorescent proteins are routinely employed as reporters in retroviral vectors. Here, we demonstrate that transduction with retroviral vectors carrying a tandem-dimer Tomato (TdTom) reporter produces two distinct fluorescent cell populations following template jumping due to a single nucleotide polymorphism between the first and second Tomato genes. Template jumping also occurs between repeated sequences in the Tomato and green fluorescent protein (GFP) genes. Thus, proper interpretation of the fluorescence intensity of transduced cells requires caution.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.02524-13

Reference22 articles.

1. Retroviral-mediated transfer of the green fluorescent protein gene into murine hematopoietic cells facilitates scoring and selection of transduced progenitors in vitro and identification of genetically modified cells in vivo;Persons DA;Blood,1997

2. Rapid identification of viable retrovirus-transduced cells using the green fluorescent protein as a marker;Klein D;Gene Ther.,1997

3. Reporter gene expression for monitoring gene transfer;Welsh S;Curr. Opin. Biotechnol.,1997

4. Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells;Cheng L;Nat. Biotechnol.,1996

5. The green fluorescent protein;Tsien RY;Annu. Rev. Biochem.,1998

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