Affiliation:
1. Environmental and Water Resources Engineering, Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, Michigan 48109-2125
Abstract
ABSTRACT
Soluble methane monooxygenase (sMMO) activity in
Methylosinus trichosporium
OB3b was found to be more strongly affected as copper-to-biomass ratios changed in a newly developed medium, M2M, which uses pyrophosphate for metal chelation, than in nitrate mineral salts (NMS), which uses EDTA. When M2M medium was amended with EDTA, sMMO activity was similar to that in NMS medium, indicating that EDTA-bound copper had lower bioavailability than pyrophosphate-bound copper. EDTA did not limit the association of copper with the cells; rather, copper was sequestered in a form which did not affect sMMO activity.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
32 articles.
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