Affiliation:
1. Observatoire Océanologique, Université Pierre et Marie Curie, CNRS UMR 7621, Institut National des Sciences de l'Univers, F-66651 Banyuls-sur-Mer Cedex,1 and
2. Agence Française de Sécurité Sanitaire des Aliments (AFSSA), 75015 Paris Cedex,2 France
Abstract
ABSTRACT
Salmonella
species are pathogenic bacteria often detected in sewage, freshwater, marine coastal water, and groundwater.
Salmonella
spp. can survive for long periods in natural waters, and the persistence of specific and epidemic strains is of great concern in public health. However, the diversity of species found in the natural environment remains unknown. The aim of this study was to investigate the diversity of
Salmonella
strains isolated from different natural aquatic systems within a Mediterranean coastal watershed (river, wastewater, and marine coastal areas). A total of 574 strains isolated from these natural environments were identified by both conventional serotyping and the ribosomal spacer-heteroduplex polymorphism (RS-HP) method (M. A. Jensen and N. Straus, PCR Methods Appl. 3:186–194, 1993). More than 40 different serotypes were found, and some serotypes probably mobilized from widespread animal-rearing activities were detected only during storm events. These serotypes may be good indicators of specific contamination sources. Furthermore, the RS-HP method based on the PCR amplification of the intergenic spacer region between the 16S and 23S rRNA genes can produce amplicon profiles allowing the discrimination of species at both serotype and intraserotype levels. This method represents a powerful tool that could be used for rapid typing of
Salmonella
isolates.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference30 articles.
1. Rapid fluorescence method for screening Salmonella spp. from enteric differential agars
2. Utilisation du bouillon Sélénite F modifié pour dénombrer Salmonella dans les milieux aquatiques.;Baleux B.;Rev. Sci. Eau.,1988
3. Salmonella spp. and fecal coliform loads in coastal waters from a point vs. nonpoint source of pollution.;Baudart J.;J. Environ. Qual.,2000
4. Media for Salmonella.;Busse M.;Int. J. Food Microbiol.,1995
5. Agricultural impacts on fecal contamination of shallow groundwaters in the Bluegrass region of Kentucky.;Coyne M. S.;Soil Sci. News Views,1994
Cited by
141 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献