Affiliation:
1. Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle/Saale, Germany
Abstract
ABSTRACT
Scald caused by
Rhynchosporium commune
is an important foliar disease of barley. Insertion mutagenesis of
R. commune
generated a nonpathogenic fungal mutant which carries the inserted plasmid in the upstream region of a gene named
PFP1
. The characteristic feature of the gene product is an Epc-N domain. This motif is also found in homologous proteins shown to be components of histone acetyltransferase (HAT) complexes of fungi and animals. Therefore, PFP1 is suggested to be the subunit of a HAT complex in
R. commune
with an essential role in the epigenetic control of fungal pathogenicity. Targeted
PFP1
disruption also yielded nonpathogenic mutants which showed wild-type-like growth
ex planta
, except for the occurrence of hyphal swellings. Complementation of the deletion mutants with the wild-type gene reestablished pathogenicity and suppressed the hyphal swellings. However, despite wild-type-level
PFP1
expression, the complementation mutants did not reach wild-type-level virulence. This indicates that the function of the protein complex and, thus, fungal virulence are influenced by a position-affected long-range control of
PFP1
expression.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Cited by
9 articles.
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