Affiliation:
1. Center for Environmental Biotechnology1 and
2. Department of Chemical Engineering,2 The University of Tennessee, Knoxville, Tennessee 37996
Abstract
ABSTRACT
Bioluminescence, mRNA levels, and toluene degradation rates in
Pseudomonas putida
TVA8 were measured as a function of various concentrations of toluene and trichloroethylene (TCE). TVA8 showed an increasing bioluminescence response to increasing TCE and toluene concentrations. Compared to uninduced TVA8 cultures,
todC1
mRNA levels increased 11-fold for TCE-treated cultures and 13-fold for toluene-treated cultures. Compared to uninduced
P. putida
F1 cultures,
todC1
mRNA levels increased 4.4-fold for TCE-induced cultures and 4.9-fold for toluene-induced cultures. Initial toluene degradation rates were linearly correlated with specific bioluminescence in TVA8 cultures.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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