Affiliation:
1. Aerosol Research and Exposure Assessment Laboratory, Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio 45267-0056
Abstract
ABSTRACT
The reactions between seven fluorogenic substrates and different groups of enzymes, esterases, lipases, phosphatases, and dehydrogenases, were studied in a search for a new method for the detection of actinomycete spores. Fluorescence measurement was chosen as a fast and sensitive method for microbial analysis. The focus of the research was on the spores of important air contaminants:
Streptomyces albus
and
Thermoactinomyces vulgaris
. For the measurement of the enzymatic activity, the chosen fluorogenic substrate was added to a mixture of spores and nutrient media, and the resulting fluorescence was measured with a spectrofluorometer. Fluorogenic substrates were found to show enzymatic activities even for dormant spores. Comparison of the enzymatic activities of dormant spores with those of vegetative cells showed similarity of the enzymatic profiles but higher activity for vegetative cells. The increase of enzymatic activity from dormant spores to vegetative cells was not linear but fluctuating. The largest fluctuations were found after 4 to 5 h of incubation. The enzymatic activities of
S. albus
were 10 to 50 times lower than those of
T. vulgaris
, except for the dehydrogenase activity, which was seven times higher. These results indicate that analysis with fluorogenic substrates has the potential for becoming a fast and sensitive method for the enumeration and identification of airborne actinomycete spores.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference21 articles.
1. American Type Culture Collection
Catalogue of bacteria and bacteriophages.
1992
American Type Culture Collection
Rockville Md
2. Protein synthesis in Bacillus stearothermophilus;Bubela B.;Biochim. Biophys. Acta,1966
3. Assessment of fungi in carpeted environments;Cole E. C.;Air Qual. Monogr.,1994
4. Stability of spores of Bacillus cereus stored on silicagel;Dufrenne J.;Int. J. Food Microbiol.,1994
5. Formation, properties and germination of actinomycete spores;Ensign J. C.;Annu. Rev. Microbiol.,1978
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