Prevalence of viridans streptococci exhibiting lactose-inhibitable coaggregation with oral actinomycetes

Abstract

Fresh oral isolates from human dental plaque were selected on the basis of their spherical morphology. In a double-blind experiment, their species identity and ability to coaggregate with human oral Actinomyces viscosus and Actinomyces naeslundii were determined. Of the 110 isolates characterized, 30 were identified as either Streptococcus mutans, Streptococcus anginosus-constellatus, or Veillonella parvula; none of these coaggregated with the actinomycetes. The remaining 80 isolates were identified as Streptococcus sanguis, Streptococcus mitis, Streptococcus MG-intermedius, or Streptococcus morbillorum. Of these 49 isolates (61%) coaggregated with actinomycetes, and nearly 90% (43 of 49 isolates) exhibited lactose-inhibitable coaggregations. Compared with previously characterized coaggregation properties determined with stock culture strains of streptococci, 82% of the fresh isolates exhibited identical coaggregations. The other 18% made up a new coaggregation group that possessed a related lactose-inhibitable coaggregation pattern. Thus, most fresh isolates that coaggregated exhibited lactose-inhibitable coaggregations with human oral actinomycetes. It is suggested that these coaggregations are mediated by a network of lectin-carbohydrate interactions similar to those already characterized in previous studies with stock cultures of actinomycetes and streptococci.