Novel Sensitive Real-Time PCR for Quantification of Bacterial 16S rRNA Genes in Plasma of HIV-Infected Patients as a Marker for Microbial Translocation-Reference-Cited by-同舟云学术

Novel Sensitive Real-Time PCR for Quantification of Bacterial 16S rRNA Genes in Plasma of HIV-Infected Patients as a Marker for Microbial Translocation

Published:2011-10 Issue:10 Volume:49 Page:3691-3693
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kramski M.¹,Gaeguta A. J.¹,Lichtfuss G. F.²³,Rajasuriar R.²³⁴,Crowe S. M.²⁵,French M. A.⁶,Lewin S. R.²³⁵,Center R. J.¹,Purcell D. F. J.¹

Affiliation:

1. Department of Microbiology and Immunology, University of Melbourne, Melbourne, Australia

2. Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Australia

3. Department of Medicine, Monash University, Melbourne, Australia

4. Faculty of Medicine, University of Malaya, Kuala Lumpur

5. Infectious Diseases Unit, Alfred Hospital, Melbourne, Australia

6. School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Australia

Abstract

ABSTRACT We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01018-11

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