Affiliation:
1. Department of Marine Sciences, University of Connecticut, Groton, Connecticut 06340
Abstract
ABSTRACT
DNA barcoding is a diagnostic technique for species identification using a short, standardized DNA. An effective DNA barcoding marker would be very helpful for unraveling the poorly understood species diversity of dinoflagellates in the natural environment. In this study, the potential utility for DNA barcoding of mitochondrial cytochrome
c
oxidase 1 (
cox1
) and cytochrome
b
(
cob
) was assessed. Among several primer sets examined, the one amplifying a 385-bp
cob
fragment was most effective for dinoflagellates. This short
cob
fragment is easy to sequence and yet possess reasonable taxon resolution. While the lack of a uniform gap between interspecific and intraspecific distances poses difficulties in establishing a phylum-wide species-discriminating distance threshold, the variability of
cob
allows recognition of species within particular lineages. The potential of this
cob
fragment as a dinoflagellate species marker was further tested by applying it to an analysis of the dinoflagellate assemblages in Long Island Sound (LIS) and Mirror Lake in Connecticut. In LIS, a highly diverse assemblage of dinoflagellates was detected. Some taxa can be identified to the species and some to the genus level, including a taxon distinctly related to the bipolar species
Polarella glacialis
, and the large number of others cannot be clearly identified, due to the inadequate database. In Mirror Lake, a
Ceratium
species and an unresolved taxon were detected, exhibiting a temporal transition from one to the other. We demonstrate that this 385-bp
cob
fragment is promising for lineage-wise dinoflagellate species identification, given an adequate database.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference46 articles.
1. Anderson, D. M. 1994. Red tides. Sci. Am.271:62-68.
2. Anderson, D. M., D. M. Kulis, G. J. Doucette, J. C. Gallagher, and E. Balech. 1994. Biogeography of toxic dinoflagellates in the genus Alexandrium from the northeastern United States and Canada. Mar. Biol.120:467-478.
3. Molecular markers: natural history and evolution. 1994
4. Balech, E. 1985. The genus Alexandrium or Gonyaulax of the tamarensis group, p. 33-38. In D. M. Anderson, A. W. White, and D. G. Baden (ed.), Toxic dinoflagellates. Elsevier, New York, NY.
5. Birky, C. W., Jr. 2007. Workshop on barcoded DNA: application to rotifer phylogeny, evolution, and systematics. Hydrobiologia593:175-183.
Cited by
81 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献