Generation of Merkel Cell Polyomavirus (MCV)-Like Particles and Their Application to Detection of MCV Antibodies-Reference-Cited by-同舟云学术

Generation of Merkel Cell Polyomavirus (MCV)-Like Particles and Their Application to Detection of MCV Antibodies

Published:2010-05 Issue:5 Volume:48 Page:1767-1770
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Touzé Antoine¹,Gaitan Julien¹,Arnold Françoise¹,Cazal Raphaël¹,Fleury Maxime J.¹,Combelas Nicolas¹,Sizaret Pierre-Yves²,Guyetant Serge³,Maruani Annabel⁴,Baay Marc⁵,Tognon Mauro⁶,Coursaget Pierre¹

Affiliation:

1. Université François Rabelais, INSERM U618, and IFR 136, Agents Transmissibles et Infectiologie, Tours, France

2. Service de Microscopie Électronique, Université François Rabelais, INSERM U966, and IFR136, Tours, France

3. Service d'Anatomie et Cytologie Pathologiques. Hôpital Trousseau, CHRU de Tours, Tours, France

4. Université François Rabelais, Service de Dermatologie, Hôpital Trousseau, CHRU de Tours, Tours, France

5. University of Antwerp, Laboratory for Cancer Research and Clinical Oncology, Universiteitsplein 1, Wilrijk, Belgium

6. School of Medicine, University of Ferrara, Ferrara, Italy

Abstract

ABSTRACT The genome of a new human polyomavirus, known as Merkel cell polyomavirus (MCV), has recently been reported to be integrated within the cellular DNA of Merkel cell carcinoma (MCC), a rare human skin cancer. To investigate MCV seroprevalence in the general population, we expressed three different MCV VP1 in insect cells using recombinant baculoviruses. Viruslike particles (VLPs) were obtained with only one of the three VP1 genes. High-titer antibodies against VP1 VLPs were detected in mice immunized with MCV VLPs, and limited cross-reactivity was observed with BK polyomavirus (BKV) and lymphotropic polyomavirus (LPV). MCV antibodies were detected in 77% of the general population, with no variations according to age.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01691-09

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