Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A-Reference-Cited by-同舟云学术

Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A

Published:1977-11 Issue:5 Volume:34 Page:518-522
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Saunders G C,Bartlett M L

Abstract

A simple double-antibody enzyme immunoassay that uses a microtechnique was developed for detecting staphylococcal enterotoxin A in food products. Sample preparation can be completed in less than 15 min. Assay sensitivity ranges from 0.4 ng (20-h test time) to 3.2 ng (1- to 3-h test time) of toxin per ml of prepared sample. Separation and detection of enterotoxin from spiked food products ranged between 72 and 98% of the amount added.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.34.5.518-522.1977

Reference16 articles.

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2. Detection of staphylococcal enterotoxin in food;Casman E. P.;Appl. Microbiol.,1965

3. A rapid solid phase radio-immunoassay for staphylococcal B enterotosin;Collins W. S.;J. Immunol.,1972

4. Enzyme-linked immunosorbent assay, ELISA. mI. Quantitation of specific antibodies by enzyme-linked anti-immunoglobulin in antigen-coated tubes;Engvall E.;J. Immunol.,1972

5. Recovery of staphylococcal enterotoxin from foods by affinity chromatography;Genigeorgis C.;Appl. Environ. Microbiol.,1976

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