Isolation and characterization of human fecal bacteria capable of 21-dehydroxylating corticoids

Author:

Bokkenheuser V D,Winter J,Dehazya P,Kelly W G

Abstract

It has been known for a decade that human intestinal flora include organisms capable of 21-dehydroxylating corticoids. Yet the identity of the organisms synthesizing 21-dehydroxylase has remained unknown. Using diluted human feces, we determined the prevalence of colonies of 21-dehydroxylating organisms on a variety of media. Isolation from the medium of colonies with the highest prevalence yielded an obligate anaerobe capable of 21-dehydroxylating deoxycorticosterone and tetrahydrodeoxycorticosterone. This transformation could be carried out in a prereduced medium by the microbial culture alone or in an aerobic medium reduced by growth of Escherichia coli. The culture shares many characteristics with Eubacterium lentum, the neotype strain of which elaborated both 21-dehydroxylase and 3alpha-hydroxysteroid dehydrogenase.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference23 articles.

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2. Metabolism of deoxycorticosterone by human fecal flora;Bokkenheuser V. D.;Appl. Microbiol.,1975

3. Formation and metabolism of tetrahydrodeoxycorticosterone by human fecal flora;Bokkenhe'user V. D.;J. Steroid Biochem.,1976

4. Dimonstrazione della conversione in vivo del desossicorticosterone in prenandiolo;Campagnoli C.;Folia Endocrinol.,1965

5. Urinary metabolites of administered corticosterone. I. Steroids liberated by glucuronidase hydrolysis;Engel L. L.;J. Biol. Chem.,1955

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