Characterization of Circular Plasmid Dimers in Borrelia burgdorferi-Reference-Cited by-同舟云学术

Characterization of Circular Plasmid Dimers in Borrelia burgdorferi

Published:1998-11 Issue:21 Volume:180 Page:5676-5681
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Tilly Kit¹,Lubke Lori²,Rosa Patricia¹

Affiliation:

1. Laboratory of Microbial Structure and Function1 and

2. Microscopy Branch,2 Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840

Abstract

ABSTRACT We have inactivated the ospC , oppAIV , and guaB genes on the 26-kb circular plasmid of Borrelia burgdorferi (cp26) by allelic exchange. On several occasions following such transformations, the cp26 of transformants had an aberrant mobility through agarose gels. Characterization of these cp26 molecules showed that the plasmid had dimerized. These dimers were quite stable during either selective or nonselective passage. Subsequent transformations with dimer DNA supported the hypothesis that in B. burgdorferi , transforming cp26 DNA most likely does not displace the resident homologous plasmid but rather must recombine in order to donate sequences that it carries. These serendipitous findings provide a mechanism for obtaining heterozygous complemented control strains when mutant phenotypes are characterized.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.180.21.5676-5681.1998

Reference35 articles.

1. Plasmid analysis of Borrelia burgdorferi, the Lyme disease agent

2. Linear DNA of Borrelia species and antigenic variation;Barbour A. G.;Trends Microbiol.,1993

3. The nucleotide sequence of a linear plasmid of Borrelia burgdorferi reveals similarities to those of circular plasmids of other prokaryotes

4. Linear plasmids of the bacterium Borrelia burgdorferi have covalently closed ends;Barbour A. G.;Science,1987

5. Linear chromosome of Borrelia burgdorferi;Baril C.;Res. Microbiol.,1989

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