Affiliation:
1. Department of Biochemistry, University of Wisconsin—Madison, Madison, Wisconsin 53706
Abstract
ABSTRACT
Tn
5
transposase (Tnp) overproduction is lethal to
Escherichia coli
. The overproduction causes cell filamentation and abnormal chromosome segregation. Here we present three lines of evidence strongly suggesting that Tnp overproduction killing is due to titration of topoisomerase I. First, a suppressor mutation of transposase overproduction killing,
stkD10
, is localized in
topA
(the gene for topoisomerase I). The
stkD10
mutant has the following characteristics: first, it has an increased abundance of topoisomerase I protein, the topoisomerase I is defective for the DNA relaxation activity, and DNA gyrase activity is reduced; second, the suppressor phenotype of a second mutation localized in
rpoH
,
stkA14
(H. Yigit and W. S. Reznikoff, J. Bacteriol. 179:1704–1713, 1997), can be explained by an increase in
topA
expression; and third, overexpression of wild-type
topA
partially suppresses the killing. Finally, topoisomerase I was found to enhance Tn
5
transposition up to 30-fold in vivo.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference59 articles.
1. The role of topoisomerase IV in partitioning bacterial replicons and the structure of catenated intermediates in DNA replication;Adams D. E.;Cell,1992
2. Berg
D. E.
Transposon Tn 5 Mobile DNA.
Berg
D. E.
Howe
M. M.
1989
185
210
American Society for Microbiology
Washington D.C
3. Berg
D. E.
Howe
M. M.
Mobile DNA.
1989
American Society for Microbiology
Washington D.C
4. E. coli XerC recombinase is required for chromosomal segregation at cell division;Blakely G.;New Biol.,1991
5. Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12;Blakely G.;Cell,1993
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献