The Escherichia coli cmlA Gene Encodes the Multidrug Efflux Pump Cmr/MdfA and Is Responsible for Isopropyl-β- d -Thiogalactopyranoside Exclusion and Spectinomycin Sensitivity-Reference-Cited by-同舟云学术

The Escherichia coli cmlA Gene Encodes the Multidrug Efflux Pump Cmr/MdfA and Is Responsible for Isopropyl-β- d -Thiogalactopyranoside Exclusion and Spectinomycin Sensitivity

Published:1998-11-15 Issue:22 Volume:180 Page:6072-6075
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Bohn Chantal¹,Bouloc Philippe¹

Affiliation:

1. Laboratoire des Réseaux de Régulations, Institut de Génétique et Microbiologie, Université Paris-Sud, CNRS/URA2225, F-91405 Orsay Cedex, France

Abstract

ABSTRACT Expression of cloned genes from isopropyl-β- d -thiogalactopyranoside (IPTG)-regulated promoters is lowered when the Escherichia coli CmlA/Cmr/MdfA efflux pump is overexpressed, probably due to IPTG exclusion from the cytoplasm. The previously reported cmlA1 mutation confers a similar phenotype. cmlA1 contains an IS 30 insertion upstream of cmr/mdfA , which creates a putative promoter. CmlA overproduction also causes spectinomycin hypersensitivity.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.180.22.6072-6075.1998

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