Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?-Reference-Cited by-同舟云学术

Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?

Published:2022-05-17 Issue:5 Volume:66 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Hinnu Mariliis¹²,Putrinš Marta¹,Kogermann Karin³,Bumann Dirk²,Tenson Tanel¹^ORCID

Affiliation:

1. University of Tartu, Institute of Technology, Tartu, Estonia

2. University of Basel, Biozentrum, Basel, Switzerland

3. University of Tartu, Institute of Pharmacy, Tartu, Estonia

Abstract

Azithromycin is a clinically important drug for treating invasive salmonellosis despite poor activity in laboratory assays for MIC. Addition of the main buffer in blood, bicarbonate, has been proposed for more physiologically relevant and more predictive testing conditions. However, we show here that bicarbonate-triggered lowering of azithromycin MIC is entirely due to alkalization of insufficiently buffered media. In addition, bicarbonate is unlikely to be altering efflux pump activity.

Funder

ERA-Net RUS Plus

Estonian Research Council

European Union, Horizon 2020, TWINNING

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/aac.02412-21

Reference17 articles.

1. Epidemiology, Clinical Presentation, Laboratory Diagnosis, Antimicrobial Resistance, and Antimicrobial Management of Invasive Salmonella Infections