A Lysine Residue Essential for Geminivirus Replication Also Controls Nuclear Localization of the Tomato Yellow Leaf Curl Virus Rep Protein-Reference-Cited by-同舟云学术

A Lysine Residue Essential for Geminivirus Replication Also Controls Nuclear Localization of the Tomato Yellow Leaf Curl Virus Rep Protein

Published:2019-05-15 Issue:10 Volume:93 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Maio Francesca¹,Arroyo-Mateos Manuel¹²,Bobay Benjamin G.³⁴⁵,Bejarano Eduardo R.²,Prins Marcel¹⁶,van den Burg Harrold A.¹

Affiliation:

1. Molecular Plant Pathology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, the Netherlands

2. Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus Teatinos, Málaga, Spain

3. Duke University NMR Center, Duke University Medical Center, Durham, North Carolina, USA

4. Department of Biochemistry, Duke University, Durham, North Carolina, USA

5. Department of Radiology, Duke University, Durham, North Carolina, USA

6. Keygene N.V., Wageningen, the Netherlands

Abstract

Nuclear activity of the replication initiator protein (Rep) of geminiviruses is essential for viral replication. We now define that one highly conserved lysine is important for nuclear import of Rep from three different begomoviruses. To our knowledge, this is the first time that nuclear localization has been mapped for any geminiviral Rep protein. Our data add another key function to this lysine residue, besides its roles in viral DNA replication and interaction with host factors, such as the SUMO E2-conjugating enzyme.

Funder

Topsector T&U

Spanish Ministerio de Cienca y Tecnologia

Keygene N.V.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01910-18

Reference97 articles.

1. Geminivirus disease complexes: an emerging threat