α-Galactosidase Aga27A, an Enzymatic Component of the Clostridium josui Cellulosome

Author:

Jindou Sadanari1,Karita Shuichi1,Fujino Emi1,Fujino Tsuchiyoshi2,Hayashi Hidenori1,Kimura Tetsuya1,Sakka Kazuo1,Ohmiya Kunio1

Affiliation:

1. Faculty of Bioresources, Mie University, Tsu 514-8507, Japan

2. Nagoya Seiraku Co. Ltd., Nagoya 468-8588, Japan

Abstract

ABSTRACT The Clostridium josui aga27A gene encodes the cellulosomal α-galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various α-galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms, especially plants. The recombinant Aga27A α-galactosidase devoid of the dockerin domain preferred highly polymeric galactomannan as a substrate to small saccharides such as melibiose and raffinose.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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