Identification of a Protein That Inactivates the Competence-Stimulating Peptide of Streptococcus pneumoniae-Reference-Cited by-同舟云学术

Identification of a Protein That Inactivates the Competence-Stimulating Peptide of Streptococcus pneumoniae

Published:2002-01-15 Issue:2 Volume:184 Page:610-613
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Bergé Mathieu¹,Langen Hanno²,Claverys Jean-Pierre¹,Martin Bernard¹

Affiliation:

1. Laboratoire de Microbiologie et Génétique Moléculaire, UMR 5100 CNRS-Université Paul Sabatier, 31062 Toulouse Cedex, France

2. Hoffmann-La Roche Ltd., CH-4070 Basel, Switzerland

Abstract

ABSTRACT Competence for genetic transformation of Streptococcus pneumoniae is a transient physiological property inducible by a competence-stimulating peptide (CSP). A 68-kDa CSP-inactivating protein was previously obtained following lithium chloride (LiCl) extraction. By the same protocol, a CSP-inactivating protein was purified and identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry as an endopeptidase, PepO. Analysis of a pepO mutant provided no support for the hypothesis that PepO participates in competence regulation. To reconcile in vitro and in vivo data, we suggest that LiCl treatment results in the release of intracellular molecules, including PepO.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.184.2.610-613.2002

Reference19 articles.

1. Alloing, G., P. de Philip, and J. P. Claverys. 1994. Three highly homologous membrane-bound lipoproteins participate in oligopeptide transport by the Ami system of the gram-positive Streptococcus pneumoniae. J. Mol. Biol.241:44–58.

2. Alloing, G., B. Martin, C. Granadel, and J. P. Claverys. 1998. Development of competence in Streptococcus pneumoniae: pheromone auto-induction and control of quorum-sensing by the oligopeptide permease. Mol. Microbiol.29:75–84.

3. Chavagnat, F., J. Meyer, and M. G. Casey. 2000. Purification, characterisation, cloning and sequencing of the gene encoding oligopeptidase PepO from Streptococcus thermophilus A. FEMS Microbiol. Lett.191:79–85.

4. Claverys, J. P., H. Prats, H. Vasseghi, and M. Gherardi. 1984. Identification of Streptococcus pneumoniae mismatch repair genes by an additive transformation approach. Mol. Gen. Genet.196:91–96.

5. Streptococcus parasanguis pepO Encodes an Endopeptidase with Structure and Activity Similar to Those of Enzymes That Modulate Peptide Receptor Signaling in Eukaryotic Cells

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