Rab5a Promotes Cytolethal Distending Toxin B-Induced Cytotoxicity and Inflammation-Reference-Cited by-同舟云学术

Rab5a Promotes Cytolethal Distending Toxin B-Induced Cytotoxicity and Inflammation

Published:2020-09-18 Issue:10 Volume:88 Page:
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Chen Ming-xian¹,Chen Yu²,Fu Rui³,Mao Guo-qun⁴,Liu Sai-yue⁵,Shen Tang-biao³^ORCID

Affiliation:

1. Department of Gastroenterology, Tongde Hospital of Zhejiang Province, Institute of Integrated Chinese and Western Medicine on Spleen-Stomach Diseases, Zhejiang Province Academy of Traditional Chinese Medicine, Hangzhou, China

2. Laboratory Animal Center, Zhejiang Province Academy of Traditional Chinese Medicine, Hangzhou, China

3. Department of Gastroenterology, Tongde Hospital of Zhejiang Province, Hangzhou, China

4. Department of Clinical Laboratory, Tongde Hospital of Zhejiang Province, Hangzhou, China

5. Department of Adverse Drug Reaction Monitoring, Zhejiang Province Center of Adverse Drug Reaction Monitoring, Hangzhou, China

Abstract

The cytolethal distending toxin B subunit (CdtB) induces significant cytotoxicity and inflammation in many cell types that are involved in the pathogenesis of postinfectious irritable bowel syndrome (PI-IBS). However, the underlying mechanisms remain unclear. This study tested the potential role of Rab small GTPase 5a (Rab5a) in the process. We tested mRNA and protein expression of proinflammatory cytokines (interleukin-1β [IL-1β] and IL-6) in THP-1 macrophages by quantitative PCR (qPCR) and enzyme-linked immunosorbent assays (ELISAs), respectively.

Funder

Zhejiang Province Institute of Science and Technology of Special Foundation

Zhejiang Province Key Research Project of Traditional Chinese Medicine

Zhejiang Medical and Healthy Science and Technology Plan Project

National Natural Science Foundation of China

Zhejiang Provincial Natural Science Foundation of China

Zhejiang Province Project of Traditional Chinese Medicine

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

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