Rapid Method for Analyzing Bacterial Behavioral Responses to Chemical Stimuli-Reference-Cited by-同舟云学术

Rapid Method for Analyzing Bacterial Behavioral Responses to Chemical Stimuli

Published:1992-07 Issue:7 Volume:58 Page:2250-2254
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Nikata Toshiyuki¹,Sumida Ken¹,Kato Junichi¹,Ohtake Hisao¹

Affiliation:

1. Department of Fermentation Technology, Hiroshima University, Higashi-Hiroshima, Hiroshima 724, Japan

Abstract

A rapid method was developed to analyze bacterial behavioral responses to chemical stimuli. Digital image processing was used to detect the accumulation of bacteria at the mouth of a capillary containing an attractant. The accumulation of bacteria was determined from the total number of cells near the mouth of the capillary per videotape frame. This method was applied to measure the chemotactic response of Pseudomonas aeruginosa cells to serine, with results similar to those obtained by the classical capillary plating assay. The videotape method is much less time-consuming and makes it possible to assess the bacterial response to an attractant within a few minutes.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.58.7.2250-2254.1992

Reference15 articles.

1. Chemotaxis in bacteria;Adler J.;Science,1966

2. Chemoreceptors in bacteria;Adler J.;Science,1969

3. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli;Adler J.;J. Gen. Microbiol.,1972

4. Chemotaxis in Eschenichia coli analysed by three-dimensional tracking;Berg H. C.;Nature (London),1972

5. Ecological aspects of microbial chemotactic behavior. Annu;Chet I.;Rev. Microbiol.,1979

Cited by 57 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Fermented botanical fertilizer controls bacterial wilt of tomatoes caused by Ralstonia pseudosolanacearum;Bioscience, Biotechnology, and Biochemistry;2024-02-21

2. Chemotactic disruption as a method to control bacterial wilt caused by Ralstonia pseudosolanacearum;Bioscience, Biotechnology, and Biochemistry;2020-12-31

3. Distinct chemotactic behavior in the original Escherichia coli K-12 depending on forward-and-backward swimming, not on run-tumble movements;Scientific Reports;2020-09-28

4. Characterization of methyl-accepting chemotaxis proteins (MCPs) for amino acids in plant-growth-promoting rhizobacterium Pseudomonas protegens CHA0 and enhancement of amino acid chemotaxis by MCP genes overexpression;Bioscience, Biotechnology, and Biochemistry;2020-09-01

5. Distinct chemotactic behavior in the originalEscherichia coliK-12 depending on forward-and-backward swimming, not on run-tumble movements;2020-02-14