SEROLOGY AND TRANSDUCTION IN STAPHYLOCOCCAL PHAGE

Author:

Dowell C. E.1,Rosenblum E. D.1

Affiliation:

1. Department of Microbiology, The University of Texas Southwestern Medical School, Dallas, Texas

Abstract

Dowell , C. E. (The University of Texas, Dallas) and E. D. Rosenblum . Serology and transduction in staphylococcal phage. J. Bacteriol. 84: 1071–1075. 1962.—A triply lysogenic strain of Staphylococcus aureus was shown to carry a serological group B phage capable of transduction. Three typing phages (53, 80, 42D), either belonging to serological group B or having a close association with it, were also shown to have transducing ability. A rapid screening method was used to isolate two new transducing phages, both of which belonged to serological group B. Propagating strain 42B/47C was found to carry a transducing phage that was neutralized by both group B and group F antisera. Nine other phages belonging to serological groups other than group B did not have generalized transducing ability, nor did three group B typing phages that were atypical in their calcium requirement. It was postulated that transducing ability is associated with staphylococcal phages of serological group B and with related phages of group F.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference21 articles.

1. The bacteriophage typing of staphylococci;BLAIR J. E.;J. Infectious Diseases,1953

2. The staphylococcal bacteriophages;BURNET F. M.;J. Pathol. Bacteriol.,1935

3. Effect of medium composition on the inhibition of staphylococcal phages. (Abstr.) Texas;DOWELL C. E.;Repts. Biol. Med.,1961

4. Transductional studies in a polylysogenic strain of Staphylococcus aureus. (Abstr.) Texas;DOWELL C. E.;Repts. Biol. Med.,1961

5. Metabolic and genetic investigations of nutritionally exacting strains of Staphylococcus pyogenes;EDGAR J. B.;Nature,1961

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