Engineering Kluyveromyces marxianus as a Robust Synthetic Biology Platform Host

Author:

Cernak Paul1,Estrela Raissa1,Poddar Snigdha12,Skerker Jeffrey M.34,Cheng Ya-Fang5,Carlson Annika K.3,Chen Berling6,Glynn Victoria M.7,Furlan Monique8,Ryan Owen W.1,Donnelly Marie K.9,Arkin Adam P.3410,Taylor John W.9,Cate Jamie H. D.1611

Affiliation:

1. Department of Molecular and Cell Biology, University of California, Berkeley, California, USA

2. Innovative Genomics Institute, University of California, Berkeley, California, USA

3. Department of Bioengineering, University of California, Berkeley, California, USA

4. Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

5. Energy Biosciences Institute, Berkeley, California, USA

6. Department of Chemistry, University of California, Berkeley, California, USA

7. Department of Environmental Science, Policy, and Management, University of California, Berkeley, California, USA

8. Department of Genetics, Evolution and Bioagents, University of Campinas, Campinas, São Paulo, Brazil

9. Department of Plant and Microbial Biology, University of California, Berkeley, California, USA

10. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

11. Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, California, USA

Abstract

The yeast Kluyveromyces marxianus grows at high temperatures and on a wide range of carbon sources, making it a promising host for industrial biotechnology to produce renewable chemicals from plant biomass feedstocks. However, major genetic engineering limitations have kept this yeast from replacing the commonly used yeast Saccharomyces cerevisiae in industrial applications. Here, we describe genetic tools for genome editing and breeding K. marxianus strains, which we use to create a new thermotolerant strain with promising fatty acid production. These results open the door to using K. marxianus as a versatile synthetic biology platform organism for industrial applications.

Funder

FAPESP

Bakar Spark Fund

CAPES Science Without Borders

National Science Foundation

Energy Biosciences Institute

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

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