Affiliation:
1. Departments of Surgery and Biometry, Louisiana State University Medical Center, New Orleans, Louisiana 70112
Abstract
The antibacterial effects of 24-h exposures to high-pressure oxygen in relation to environmental CO
2
were studied at 3 atm absolute (ata) and at 1 ata. Eight gram-negative, aerobic and facultatively aerobic, pathogenic enteric bacteria (
Salmonella typhosa, Salmonella paratyphi, Salmonella schottmuelleri, Shigella dysenteriae, Shigella flexneri, Proteus vulgaris, Pseudomonas aeruginosa
, and
Escherichia coli
) were exposed as shallow-broth cultures and agar surface cultures. Although broths supplemented with 0.2% glucose permitted some growth of
Salmonella typhosa, Salmonella schottmuelleri, Shigella dysenteriae
, and
Shigella flexneri
during exposure to high-pressure oxygen in the presence of CO
2
, the other species grew only after the exposure, indicating a bacteriostatic effect. Both bacteriostatic and bactericidal effects were demonstrated on the surface of Trypticase soy agar, where killing of
Salmonellea typhosa, Proteus vulgaris
, and
Pseudomonas aeruginosa
was significantly greater after exposure to pure O
2
at 3 ata than at 1 ata. At 3 ata, significantly more killing occurred upon exposure of all species (except
Shigella dysenteriae
and
S. flexneri
) on an agar surface to 100% O
2
as compared with exposure to a mixture of 95% O
2
+ 5% CO
2
. Thus, deprivation of CO
2
during exposure to pure O
2
enhanced the bactericidal effect of high-pressure oxygen.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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