Affiliation:
1. National Institute for Leprosy Research, Tokyo, Japan
2. Institute for Protein Research and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
Abstract
Kusaka, Takashi
(National Institute for Leprosy Research, Tokyo, Japan),
Ryo Sato, and Ko Shoji
. Comparison of cytochromes in mycobacteria grown in vitro and in vivo. J. Bacteriol.
87:
1383–1388. 1964.—Spectrophotometric investigations were made on cell suspensions or particulate fractions of four species of mycobacteria which had been cultivated in vitro. The results obtained indicated the presence in
Mycobacterium smegmatis
of cytochromes of the
a, b
1
, and
c
types, as well as a CO-binding pigment similar to cytochrome
o
; in BCG and
M. avium
, cytochromes of the
a, b
, and
c
types were present; and, in
M. paratuberculosis
, cytochromes of the
a, b
1
, and
c
types were present. Although no clear interrelations were evident between the cytochrome patterns of these organisms and the ease with which they can be cultivated in vitro, it was found that the total reduced nicotinamide adenine dinucleotide oxidase activity of the particulate fraction was remarkably low in
M. paratuberculosis
, which can be grown in vitro only with great difficulty. The cells of BCG grown in vivo and those of
M. lepraemurium
isolated from leprous nodules of mice were found to be completely deficient in cytochrome pigments.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
26 articles.
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