Modulation of humoral response to a 12-amino-acid site on the poliovirus virion

Author:

Icenogle J P,Minor P D,Ferguson M,Hogle J M

Abstract

Most monoclonal antibodies to poliovirus 3 but not poliovirus 1 require a single 12-amino-acid sequence in virion protein VP1 for neutralization (site 1). None of the available monoclonal antibodies requiring this site bound virions after tryptic cleavage of site 1. This result allowed the amount of site 1-specific antibodies to be determined in an antiserum by comparing its reactivity with virus and trypsin-cleaved virus. Antisera to poliovirus 3 Sabin strain (PS3) but not poliovirus 1 Sabin showed site 1 immunodominance, consistent with the frequency of isolation of site 1-specific monoclonal antibodies to these viruses. Cleavage of site 1 prior to immunization dramatically reduced the immunogenicity of this site in PS3. However, the antiserum against trypsin-cleaved PS3 still had a high neutralization titer, demonstrating that sites other than site 1 can elicit a neutralizing response to PS3. Other antisera to PS3 showed significant variability in the response to site 1, indicating that other factors, such as the genetic background of inbred mouse strains, the species immunized, and the immunization protocol, also affect immunodominance. In particular, a serum from a human infant recently immunized with oral trivalent vaccine had little response to site 1.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference17 articles.

1. Mutations conferring resistance to neutralization with monoclonal antibodies in type 1 poliovirus can be located outside or inside the antibodybinding site;Blondel B.;J. Virol.,1986

2. Antigenic variation and resistance to neutralization in poliovirus type 1;Diamond D. C.;Science,1985

3. Trypsin sensitivity of the Sabin strain of type 1 poliovirus: cleavage sites in virions and related particles;Fricks C. E.;J. Virol.,1985

4. Three-dimensional structure of poliovirus at 2.9 A resolution;Hogle J. M.;Science,1985

5. Hogle J. M. M. Chow and D. J. Filman. 1986. High resolution structure of poliovirus: three-dimensional distribution of antigenic sites in the intact virus p. 3-13. In R. M. Chanock R. A. Lerner and F. Brown (ed.) Vaccines 86. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

Cited by 65 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Host Immune Responses to Enterovirus Infections;Human Enterovirus Infections;2014-04-09

2. Structure determination of enterovirus 71;Acta Crystallographica Section D Biological Crystallography;2012-08-18

3. Mapping B-cell epitopes in equine rhinitis B viruses and identification of a neutralising site in the VP1 C-terminus;Veterinary Microbiology;2012-03

4. Interaction of Decay-Accelerating Factor with Echovirus 7;Journal of Virology;2010-12-15

5. Repertoire of antibodies against type 1 poliovirus in human sera;Journal of Virological Methods;2010-11

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3