Roles of rpoN , fliA, and flgR in Expression of Flagella in Campylobacter jejuni

Abstract

ABSTRACT Three potential regulators of flagellar expression present in the genome sequence of Campylobacter jejuni NCTC 11168, the genes rpoN , flgR, and fliA, which encode the alternative sigma factor ς 54 , the ς 54 -associated transcriptional activator FlgR, and the flagellar sigma factor ς 28 , respectively, were investigated for their role in global regulation of flagellar expression. The three genes were insertionally inactivated in C. jejuni strains NCTC 11168 and NCTC 11828. Electron microscopic studies of the wild-type and mutant strains showed that the rpoN and flgR mutants were nonflagellate and that the fliA mutant had truncated flagella. Immunoblotting experiments with the three mutants confirmed the roles of rpoN , flgR, and fliA in the expression of flagellin.