Identification of Nucleotide Sequences for the Specific and Rapid Detection of Yersinia pestis-Reference-Cited by-同舟云学术

Identification of Nucleotide Sequences for the Specific and Rapid Detection of Yersinia pestis

Published:2001-08 Issue:8 Volume:67 Page:3759-3762
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Radnedge Lyndsay¹,Gamez-Chin Silvia¹,McCready Paula M.¹,Worsham Patricia L.²,Andersen Gary L.¹

Affiliation:

1. Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California 94551,1 and

2. United States Army Research Institute of Infectious Diseases, Fort Detrick, Maryland 217022

Abstract

ABSTRACT Suppression subtractive hybridization, a cost-effective approach for targeting unique DNA, was used to identify a 41.7-kb Yersinia pestis -specific region. One primer pair designed from this region amplified PCR products from natural isolates of Y. pestis and produced no false positives for near neighbors, an important criterion for unambiguous bacterial identification.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.67.8.3759-3762.2001

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