Affiliation:
1. Dipartimento Scientifico e Tecnologico, Facoltà di Scienze MM.FF.NN., Università degli Studi di Verona, 37134 Verona,1 and
2. DOFATA, Università degli Studi di Catania, 95124 Catania,2 Italy
Abstract
ABSTRACT
In this study, we succeeded in differentiating
Lactobacillus plantarum,
Lactobacillus pentosus,
and
Lactobacillus paraplantarum
by means of
recA
gene sequence comparison. Short homologous regions of about 360 bp were amplified by PCR with degenerate consensus primers, sequenced, and analyzed, and 322 bp were considered for the inference of phylogenetic trees. Phylograms, obtained by parsimony, maximum likelihood, and analysis of data matrices with the neighbor-joining model, were coherent and clearly separated the three species. The validity of the
recA
gene and RecA protein as phylogenetic markers is discussed. Based on the same sequences, species-specific primers were designed, and a multiplex PCR protocol for the simultaneous distinction of these bacteria was optimized. The sizes of the amplicons were 318 bp for
L. plantarum
, 218 bp for
L. pentosus,
and 107 bp for
L. paraplantarum
. This strategy permitted the unambiguous identification of strains belonging to
L. plantarum,
L. pentosus,
and
L. paraplantarum
in a single reaction, indicating its applicability to the speciation of isolates of the
L. plantarum
group.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
516 articles.
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