Purification and Characterization of a Dimethoate-Degrading Enzyme of Aspergillus niger ZHY256, Isolated from Sewage-Reference-Cited by-同舟云学术

Purification and Characterization of a Dimethoate-Degrading Enzyme of Aspergillus niger ZHY256, Isolated from Sewage

Published:2001-08 Issue:8 Volume:67 Page:3746-3749
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Liu Yu-Huan¹,Chung Ying-Cheng¹,Xiong Ya²

Affiliation:

1. State Key Laboratory for Biocontrol, School of Life Science,1 and

2. School of Chemistry and Chemical Engineering,2 Zhong Shan University, Guang Zhou 510275, People's Republic of China

Abstract

ABSTRACT A dimethoate-degrading enzyme from Aspergillus niger ZHY256 was purified to homogeneity with a specific activity of 227.6 U/mg of protein. The molecular mass of the purified enzyme was estimated to be 66 kDa by gel filtration and 67 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The isoelectric point was found to be 5.4, and the enzyme activity was optimal at 50°C and pH 7.0. The activity was inhibited by most of the metal ions and reagents, while it was induced by Cu 2+ . The Michaelis constant ( K m ) and V max for dimethoate were 1.25 mM and 292 μmol min −1 mg of protein −1 , respectively.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.67.8.3746-3749.2001

Reference21 articles.

1. Properties of β-glucosidase purified from Clostridium thermocellum.;Ait N.;J. Gen. Microbiol.,1982

2. Estimation of the molecular weights of proteins by Sephadex gel filtration.;Andrew P.;Biochem. J.,1965

3. AOAC International Official methods of analysis 15th ed. section 970.52 K (a)(1). 1990 AOAC International Arlington Va

4. Purification and Characterization of Cystathionine (gamma)-Lyase from Lactococcus lactis subsp. cremoris SK11: Possible Role in Flavor Compound Formation during Cheese Maturation

5. Gas chromatography of pesticides.;Coulson D. M.;Adv. Pest Control Res.,1962

Cited by 75 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Developments and perspectives on microbiology of Sun Yat-sen University;SCIENTIA SINICA Vitae;2024-09-01

2. Dimethoate-induced toxicity: a comprehensive review of its aquatic environmental hazards;Toxicology and Environmental Health Sciences;2024-08-15

3. Chlorpyrifos enrichment enhances tolerance of Anabaena sp. PCC 7119 to dimethoate;Environmental Research;2024-05

4. Accelerated Degradation of four Organophosphorus Insecticides by Malathion Tolerant Aspergillus niger MRU01 a Soil Fungus;Geomicrobiology Journal;2023-09-21

5. Organophosphate pesticides an emerging environmental contaminant: Pollution, toxicity, bioremediation progress, and remaining challenges;Journal of Environmental Sciences;2023-05